There is increasing tendency of the hospital infection, and the incidence of the gram negative organisms are steadly increased as their causative agents. Among them Pseudomonas aeruginosa is one of the most important and frequently encountered pathogens recently due to rapid emergence of resistant strain especially in immunologically compromised patients.
An experimental study of the effects of ´Pseudomonas polyvalent vaccine has been performed for their survival rates and histopathologic lesions of various parenchymal organs with intraperitoneal inoculation of Pseudomonas aeruginosa in ICR mice.
The polyvalent vaccine was prepared with heat killed whole cells of 10 serotypes of Pseudomonas aeruginosa, which were isolated from the Dept. of Microbiology, Kyung Hee School of Medicine and Seoul National University, and they were devided into type I & II.
The infection was made by intraperitoneal inoculation of Pseudomonas aeruginosa (serotype, F, inoculum size 0.1 ml), immediately after 2nd degree cutaneous bums.
The results were as follows:
1. The survival rate of the immune mice was 92% and that of the non-immune mice was 26%.
2. The histopathologic findings of the liver in the non-immune mice were severe congestion, Kupffer cell reaction, parenchymal necrosis and portal inflammation in most of them, and those, in the immune mice were reactive changes without significant parenchymal alterations.
3. The histopathologic findings of the spleen were reactive changes in both immune and non-immune mice.
4. The histopathologic findings of the lung in the non-immune mice were severe congestion, hemorrhage,
emphysematous change & septic inflammation in most of them and those in the immune mice were
mild congestion & hemorrhage with emphysematous change in small numbers.
5. The histopathologic findings of the heart in non-immune mice were severe congestion, septic inflamma
tion (about 70%) & thrombus formation (about 40%) and those in immune mice were congestion alone.
6. The histopathologic findings of the kindney in non-immune mice were severe congestion in all, heomorr-
hage (about 30%) & interstitial inflammation (about 50%) and those in immune mice were congestion alone.
With the above results we can suspect there is a significant protective effects of the Polyvalent pseudomonas vaccine in the pseudomonas sepsis induced by intraperitoneal inoculation in ICR mice.
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